Targeted capture | 合法醫療器材資訊網

由FZakrzewski著作·2019·被引用15次—Here,wecomparethecommonlyusedmultiplexPCR-basedNGSapproach(BRCADNARepairPanel-TruSeqCustomAmpliconLowInputpanel(IlluminaInc ...

Quality improvement of BRCA1/2 panel NGS of FFPE-sample derived DNA by adjusted targeted capture-based enrichment

We routinely apply the TruSight Cancer Panel (Illumina, Inc.) for germline variant analysis in HBOC families. Library preparation includes a so called tagmentation step in which high-quality, blood-derived DNA is sheared by a transposase and a capture step enriching 94 cancer-associated genes including BRCA1/2. In order to apply the 94 gene panel to low quality FFPE samples, we implemented an adapted strategy that relies on random fragmentation of DNA using the Covaris ultrasonicator (Covaris, Inc.) followed by application of the TruSeq DNA Nano kit (Illumina, Inc.) prior to enrichment via the TruSight Cancer Panel. The tagmentation step, which is usually included in the TruSighCancer preparation protocol, was skipped. To compare the performance of the modified targeted capture-based protocol to a ...

PCR for NGS | 合法醫療器材資訊網

PCR | 合法醫療器材資訊網

See how to enrich your targets using PCR-based capture technology in NGS applications. Read More

Targeted PCR | 合法醫療器材資訊網

由 MA Jones 著作 · 2011 · 被引用 3 次 — The purpose of this study was to improve molecular diagnosis for CDG by developing and validating a next generation sequencing (NGS) panel for comprehensive ... Read More

A Next | 合法醫療器材資訊網

由 YO Alekseyev 著作 · 2018 · 被引用 42 次 — This primer is written to provide an introduction to NGS for those ... Quantitative PCR, An extremely sensitive PCR-based laboratory ... Read More

PCR Techniques in Next | 合法醫療器材資訊網

由 RS Goswami 著作 · 2016 · 被引用 2 次 — Although NGS has rapidly become an important part of clinical molecular diagnostics, whole genome sequencing is still difficult to implement in a clinical ... Read More

Simple multiplexed PCR | 合法醫療器材資訊網

由 A Ståhlberg 著作 · 2017 · 被引用 70 次 — Ståhlberg et al. describe SiMSen-seq, a barcoding NGS library preparation approach to enable detection of rare variant alleles from as ... Read More

MIG-seq: an effective PCR | 合法醫療器材資訊網

由 Y Suyama 著作 · 2015 · 被引用 125 次 — The recent development of next-generation sequencing (NGS) technology has allowed the effective discovery and genotyping of large numbers of ... Read More

Longrange PCR | 合法醫療器材資訊網

由 O Zakerska-Banaszak 著作 · 2017 · 被引用 5 次 — After the design and optimization of long range PCR-based ... Next-generation sequencing (NGS) technology has become currently an essential ... Read More

Targeted capture | 合法醫療器材資訊網

由 F Zakrzewski 著作 · 2019 · 被引用 15 次 — Here, we compare the commonly used multiplex PCR-based NGS approach (BRCA DNA Repair Panel - TruSeq Custom Amplicon LowInput panel (Illumina Inc ... Read More

NGS vs. qPCR | 合法醫療器材資訊網

While qRT-PCR is useful for quantifying the expression of a few genes, it can only detect known sequences. In contrast, RNA sequencing (RNA-Seq) using NGS ... Read More

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