PCR for NGS | 合法醫療器材資訊網

Next-generation polymerases for next-generation sequencing

The Polymerase Chain Reaction (PCR) is acknowledged as one of the most enabling technologies in molecular biology.1 The advent of next-generation sequencing (NGS) paved the way for a plethora of new PCR applications. With the exception of PCR-free protocols (which have become the gold standard for human whole-genome sequencing), the majority of strategies used to convert samples to sequencing-ready NGS libraries involve one or more PCR steps. In addition, PCR-based methods are used at other stages of the sample prep process, including quality control and/or quantification steps.

NGS brings many challenges to PCR technology. These include limited amounts of template, and low or variable sample quality. Library amplification steps require true (unbiased) replication of highly complex populations of molecules. Extreme...

PCR for NGS | 合法醫療器材資訊網

PCR | 合法醫療器材資訊網

See how to enrich your targets using PCR-based capture technology in NGS applications. Read More

Targeted PCR | 合法醫療器材資訊網

由 MA Jones 著作 · 2011 · 被引用 3 次 — The purpose of this study was to improve molecular diagnosis for CDG by developing and validating a next generation sequencing (NGS) panel for comprehensive ... Read More

A Next | 合法醫療器材資訊網

由 YO Alekseyev 著作 · 2018 · 被引用 42 次 — This primer is written to provide an introduction to NGS for those ... Quantitative PCR, An extremely sensitive PCR-based laboratory ... Read More

PCR Techniques in Next | 合法醫療器材資訊網

由 RS Goswami 著作 · 2016 · 被引用 2 次 — Although NGS has rapidly become an important part of clinical molecular diagnostics, whole genome sequencing is still difficult to implement in a clinical ... Read More

Simple multiplexed PCR | 合法醫療器材資訊網

由 A Ståhlberg 著作 · 2017 · 被引用 70 次 — Ståhlberg et al. describe SiMSen-seq, a barcoding NGS library preparation approach to enable detection of rare variant alleles from as ... Read More

MIG-seq: an effective PCR | 合法醫療器材資訊網

由 Y Suyama 著作 · 2015 · 被引用 125 次 — The recent development of next-generation sequencing (NGS) technology has allowed the effective discovery and genotyping of large numbers of ... Read More

Longrange PCR | 合法醫療器材資訊網

由 O Zakerska-Banaszak 著作 · 2017 · 被引用 5 次 — After the design and optimization of long range PCR-based ... Next-generation sequencing (NGS) technology has become currently an essential ... Read More

Targeted capture | 合法醫療器材資訊網

由 F Zakrzewski 著作 · 2019 · 被引用 15 次 — Here, we compare the commonly used multiplex PCR-based NGS approach (BRCA DNA Repair Panel - TruSeq Custom Amplicon LowInput panel (Illumina Inc ... Read More

NGS vs. qPCR | 合法醫療器材資訊網

While qRT-PCR is useful for quantifying the expression of a few genes, it can only detect known sequences. In contrast, RNA sequencing (RNA-Seq) using NGS ... Read More

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