MIG-seq: an effective PCR | 合法醫療器材資訊網

由YSuyama著作·2015·被引用125次—Therecentdevelopmentofnext-generationsequencing(NGS)technologyhasallowedtheeffectivediscoveryandgenotypingoflargenumbersof ...

Template DNA

Total DNAs were extracted using suitable methods for each species from various amounts of material ranging from 6 μg (Calanoida copepod) to 50 mg of each sample (Supplementary Table 4[1]). Approximately 2–100 ng of DNA was used for the 1st PCR as template DNA. Strict quantification and equalization of the sample DNA was not applied because heterogeneous amplification among the samples could be adjusted at the multiplexing step after the 2nd PCR.

Primer selection

Several steps were conducted to select the MIG-seq primers as follows: 1) For the two-base anchor sequences, only AC, AG, CC, GG, TC and TG combinations were selected, because C or G is suitable for the 3′ ends of the primers and CG/GC is not suitable because of reverse complementarity. 2) For the three-base core motifs (sequences) that comprised three different bases (e.g. ACT), all combinations (4 × 3 × 2 = 24)...

PCR for NGS | 合法醫療器材資訊網

PCR | 合法醫療器材資訊網

See how to enrich your targets using PCR-based capture technology in NGS applications. Read More

Targeted PCR | 合法醫療器材資訊網

由 MA Jones 著作 · 2011 · 被引用 3 次 — The purpose of this study was to improve molecular diagnosis for CDG by developing and validating a next generation sequencing (NGS) panel for comprehensive ... Read More

A Next | 合法醫療器材資訊網

由 YO Alekseyev 著作 · 2018 · 被引用 42 次 — This primer is written to provide an introduction to NGS for those ... Quantitative PCR, An extremely sensitive PCR-based laboratory ... Read More

PCR Techniques in Next | 合法醫療器材資訊網

由 RS Goswami 著作 · 2016 · 被引用 2 次 — Although NGS has rapidly become an important part of clinical molecular diagnostics, whole genome sequencing is still difficult to implement in a clinical ... Read More

Simple multiplexed PCR | 合法醫療器材資訊網

由 A Ståhlberg 著作 · 2017 · 被引用 70 次 — Ståhlberg et al. describe SiMSen-seq, a barcoding NGS library preparation approach to enable detection of rare variant alleles from as ... Read More

MIG-seq: an effective PCR | 合法醫療器材資訊網

由 Y Suyama 著作 · 2015 · 被引用 125 次 — The recent development of next-generation sequencing (NGS) technology has allowed the effective discovery and genotyping of large numbers of ... Read More

Longrange PCR | 合法醫療器材資訊網

由 O Zakerska-Banaszak 著作 · 2017 · 被引用 5 次 — After the design and optimization of long range PCR-based ... Next-generation sequencing (NGS) technology has become currently an essential ... Read More

Targeted capture | 合法醫療器材資訊網

由 F Zakrzewski 著作 · 2019 · 被引用 15 次 — Here, we compare the commonly used multiplex PCR-based NGS approach (BRCA DNA Repair Panel - TruSeq Custom Amplicon LowInput panel (Illumina Inc ... Read More

NGS vs. qPCR | 合法醫療器材資訊網

While qRT-PCR is useful for quantifying the expression of a few genes, it can only detect known sequences. In contrast, RNA sequencing (RNA-Seq) using NGS ... Read More

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