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Isolation of Genomic DNA from Mouse Tails | 合法醫療器材資訊網

Isolation of Genomic DNA from Mouse Tails

Protocol·由TZangala著作·2007·被引用23次—Protocol·Cuttailpieces(3mm)andmarkears.·Add720mlSTEand30mlProteinaseK(10mg/mlstock).·Incubateat55°Conheatingblockand ...

 Isolation of Genomic DNA from Mouse Tails

Cut tail pieces (3mm) and mark ears.

Add 720 ml STE and 30 ml Proteinase K (10 mg/ml stock).

Incubate at 55°C on heating block and vortex every hour for 3 hours at top speed for 10 seconds.

Inactivate proteinase K at 70°C for 5 minutes.

Quench on ice for 5 minutes.

Centrifuge tail DNA for 10 minutes at full speed.

Decant into new tube containing 720 ml Isopropanol. 

Precipitate DNA by inverting the tube or vortexing.

Spin down DNA for 5 minutes at full speed. Remove supernatant.

Wash pellet with 70% ethanol.

Spin down genomic DNA 5 minutes at full spe...

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DNA | 合法醫療器材資訊網
DNA Isolation Protocols · 1. Remove 0.5 mm of tail into polypropylene microfuge tube (do not mince). · 2. Add 0.5 ml DNA digestion buffer with proteinase K added ... Read More
Mouse Tail Extraction with Proteinase K Protocol | 合法醫療器材資訊網
2019年3月6日 — Introduction. Clean genomic mouse DNA is used for the purposes of genotyping and molecular cloning. Mice are often used as a model organism ... Read More
DNA Extraction from Mouse Tail (Manual) | 合法醫療器材資訊網
http://www.abnova.com ) - DNA extraction is a routine procedure to collect DNA for subsequent molecular ... Read More
Extraction of DNA from mouse tails | 合法醫療器材資訊網
由 Z Wang 著作 · 2006 · 被引用 60 次 — With this method, one can routinely isolate approximately 200 samples/day from mouse tissues. The simplicity of the procedure and the quality of ... Read More
Benchmarks | 合法醫療器材資訊網
由 Z Wang 著作 · 2006 · 被引用 60 次 — Protocol for Genomic DNA Isolation from Mouse Tails. 1. 2. 3. 4. 5. 6. Digest approximately 2 mm mouse tail with 300 μL digestion buffer [5 mM EDTA, pH. Read More
Genomic DNA Isolation Protocol | 合法醫療器材資訊網
B. DNA Isolation ... Note: For mouse tail or animal tissue lysates, elution volume will be approximately 500 μL. For tissue culture cell lysates, elution volume ... Read More
Tail DNA Purification Protocol | 合法醫療器材資訊網
Protocol for isolating High Molecular Weight DNA from mouse tails · 1. Cut 0.5 cm of tail and place in a 1.5 ml microfuge tube (tails can be frozen at -20°C ... Read More
Isolation of Genomic DNA from Mouse Tails | 合法醫療器材資訊網
Protocol · 由 T Zangala 著作 · 2007 · 被引用 23 次 — Protocol · Cut tail pieces (3mm) and mark ears. · Add 720 ml STE and 30 ml Proteinase K (10 mg/ml stock). · Incubate at 55°C on heating block and ... Read More
DNA Preparation Techniques | 合法醫療器材資訊網
Mouse genomic DNA for genotyping — All other mice are biopsied via tail clips. A tail sample of only 1-2 mm is needed when doing PCR, but about 5 mm ... Read More
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