Mouse Tail Extraction with Proteinase K Protocol | 合法醫療器材資訊網

DNA Isolation Protocols · 1. Remove 0.5 mm of tail into polypropylene microfuge tube (do not mince). · 2. Add 0.5 ml DNA digestion buffer with proteinase K added ... Read More

2019年3月6日 — Introduction. Clean genomic mouse DNA is used for the purposes of genotyping and molecular cloning. Mice are often used as a model organism ... Read More

http://www.abnova.com ) - DNA extraction is a routine procedure to collect DNA for subsequent molecular ... Read More

由 Z Wang 著作 · 2006 · 被引用 60 次 — With this method, one can routinely isolate approximately 200 samples/day from mouse tissues. The simplicity of the procedure and the quality of ... Read More

由 Z Wang 著作 · 2006 · 被引用 60 次 — Protocol for Genomic DNA Isolation from Mouse Tails. 1. 2. 3. 4. 5. 6. Digest approximately 2 mm mouse tail with 300 μL digestion buffer [5 mM EDTA, pH. Read More

B. DNA Isolation ... Note: For mouse tail or animal tissue lysates, elution volume will be approximately 500 μL. For tissue culture cell lysates, elution volume ... Read More

Protocol for isolating High Molecular Weight DNA from mouse tails · 1. Cut 0.5 cm of tail and place in a 1.5 ml microfuge tube (tails can be frozen at -20°C ... Read More

Protocol · 由 T Zangala 著作 · 2007 · 被引用 23 次 — Protocol · Cut tail pieces (3mm) and mark ears. · Add 720 ml STE and 30 ml Proteinase K (10 mg/ml stock). · Incubate at 55°C on heating block and ... Read More

Mouse genomic DNA for genotyping — All other mice are biopsied via tail clips. A tail sample of only 1-2 mm is needed when doing PCR, but about 5 mm ... Read More