DNA | 合法醫療器材資訊網

DNAIsolationProtocols·1.Remove0.5mmoftailintopolypropylenemicrofugetube(donotmince).·2.Add0.5mlDNAdigestionbufferwithproteinaseKadded ...

This protocol yields a highly purified DNA preparation from mouse tail biopsies.

1. Remove 0.5 mm of tail into polypropylene microfuge tube (do not mince). (The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.)

2. Add 0.5 ml DNA digestion buffer with proteinase K added to 0.5 mg/ml final concentration. (0.5 mg/ml is a high concentration and can probably be reduced.)

DNA digestion buffer:

3. Incubate overnight at 50-55 °C with gentle shaking. (At this step, mechanical agitation greatly aids complete disruption of the tail.)

4. Quick-spin tubes to get solution off inside of cap.

5. Fill inside well of microfuge tube cap with vacuum grease. (We use Dow Corning high-vacuum grease and a 10cc syringe to disp...