Hybridization capture vs amplicon sequencing | 合法醫療器材資訊網

2019年11月21日—NGShybridizationcapturevsampliconsequencing:application-dependentconsiderationsforchoosingwhichtargetedsequencingmethodisbest ...

Next generation sequencing[1] (NGS) is a revolutionary technique that has found wide applicability, including determining and characterizing unknown sequences, assessing changes and variability in known sequences, and quantifying gene expression. High-throughput sequencing enables sequence profiling of DNA and RNA and can run samples in parallel, resulting in massive time savings. The ability to generate large amounts of sequencing data in a relatively short amount of time supports a wide range of genetic analysis applications and accelerates advances in fundamental and applied research.

To even further decrease time and cost associated with sequencing, targeted NGS[2] gives users the opportunity to sequence only specific areas of interest of the genome while omitting regions irrelevant t...