Targeted next generation sequencing by hybridization capture ... | 合法醫療器材資訊網

Hybridizationcapture,alsocalledtargetenrichment,isamethodoftargetednextgenerationsequencing(othermethodsoftargetedsequencingcanincludethe ...

Hybridization capture, also called target enrichment, is one method of targeted next generation sequencing[1] (other methods include the use of amplicons or molecular inversion probes). Before performing hybridization capture, DNA samples are converted into sequencing libraries[2]—a process that involves random shearing of DNA and ligation of sequencing adaptors[3] to the ends of the resulting fragments (PCR amplification may or may not be required). Regions of interest within the library are then captured using long, biotinylated oligonucleotide baits (probes).

Biotinylated baits are designed to hybridize to regions of interest (e.g., exome, known genes related to cancer or a metabolic pathway, etc.). After bait hybridization, separation occurs via magnet with the streptavidin-biotin binding complex.

Baits can be arranged...