Hybrid Capture | 合法醫療器材資訊網

由MGaudin著作·2018·被引用26次—Thisreviewdescribestarget-enrichmentapproachesfollowedbynextgenerationsequencingandtheirrecentapplicationtotheresearchand ...

The development of next-generation sequencing (NGS) approaches has revolutionized human clinical research because of its ability to rapidly generate large volumes of sequencing data per run, with a concomitant decrease of sequencing costs (Shendure and Ji, 2008[1]). Unbiased ultra-deep sequencing of complex samples is now accessible, although bioinformatics analyses may still be long and tedious. This issue is particularly problematic in the field of infectious disease diagnostic, where the rapid identification and functional characterization of a particular pathogen is critical for the clinical management of infected patients. So far, polymerase chain reaction (PCR) has been the gold standard method for the clinical diagnosis of infectious diseases (Edwards and Gibbs, 1994[2]). This approach, which is based on the amplification of a generally short and conserved genomic...