DNA Extraction and Purification | 合法醫療器材資訊網

ChoosingthecorrectDNAextractionkitcansavecrucialtimeonoptimization...microsatelliteanalysis,SNPgenotyping,andquantitative,real-timePCR.

Introduction

Basic considerations

DNA extraction is required for a variety of molecular biology applications. Figure 1 lists the basic steps involved in all DNA extraction methods. Many commercial kits are available to isolate DNA from a variety of biological materials [1, 2]. The sensitivity of polymerase chain reaction (PCR) detection has been shown to be different for various DNA kits [3]. Therefore, selecting the best methodology for your application is crucial.

Figure 1. Basic steps involved in all DNA extraction methods.

Choosing the correct DNA extraction kit can save crucial time on optimization and execution of the experiment. Factors to be considered for selecting a kit include: