An Improved DNA Extraction Method for Efficient and ... | 合法醫療器材資訊網

由JYuan著作·2015·被引用44次—Whilequantitativemoleculartechniqueshavebeenincreasinglyusedinphytoplanktonresearch,aneffectiveandsimplemethodbroadlyapplicable ...

Marine phytoplankton are highly diverse with different species possessing different cell coverings, posing challenges for thoroughly breaking the cells in DNA extraction yet preserving DNA integrity. While quantitative molecular techniques have been increasingly used in phytoplankton research, an effective and simple method broadly applicable to different lineages and natural assemblages is still lacking. In this study, we developed a bead-beating protocol based on our previous experience and tested it against 9 species of phytoplankton representing different lineages and different cell covering rigidities. We found the bead-beating method enhanced the final yield of DNA (highest as 2 folds) in comparison with the non-bead-beating method, while also preserving the DNA integrity. When our method was applied to a f...