Retinal regeneration is facilitated by the presence of surviving ... | 合法醫療器材資訊網

由TSherpa著作·2014·被引用43次—Keywords:opticnervehead;photoreceptors;regeneration;retina;retinalganglioncells;rosettes;sonichedgehog;zebrafish.©2014WileyPeriodicals,Inc.

Figure 7

Distribution of Hh protein in undamaged and regenerating zebrafish retinas. A . Western…

Distribution of Hh protein in undamaged and regenerating zebrafish retinas. A. Western blotting results using the anti-Shh antibody from AnaSpec (AnaSpec, Fremont, CA; #55574). Lane 1, proteins derived from zebrafish embryos; lane 2, molecular size ladder. Proteins in lane 1 likely correspond to unprocessed (50kDa) and processed (20kDa) Hh protein. B. In a 4 day old larval zebrafish eye, anti-Shh antibody stains the retinal pigmented epithelium (RPE), the outer plexiform layer (OPL), the inner plexiform layer (IPL), the nerve fiber layer (NFL), and extracellular regions of the circumferential germinal zone (CGZ). C. Same as (B), but with DAPI staining to show locations of nuclear layers. OLM, outer limiting membrane; ONL, outer nuclear layer; ...