Detection Systems in Immunohistochemistry | 合法醫療器材資訊網

由SShojaeian著作·2018·被引用1次—2.Directmethod...Directdetectionmethodsareknownasaone-stepprocessapplyingaprimaryantibody,whichisdirectlylabeledwithreportermolecules,such ...

Immunohistochemistry (IHC) represents a way to build a picture of particular distribution and localization of molecular markers within cells and in the proper tissue context and is a powerful tool that provides important diagnostic, prognostic, and predictive information supplemental to the morphological assessment of the tissues. Although less sensitive quantitatively than such immunoassays as western blotting or ELISA, IHC enables observation of molecular signature in the context of intact tissue. In its very simplified method, IHC visualizes target antigens by using target-specific antibodies tagged with appropriate labels. However, lack of need for labeling of molecular marker-specific primary antibodies and higher sensitivity made indirect staining methods as the preferred staining method. The need for more sensitive detection systems in cas...