Direct vs indirect detection in IHC | 合法醫療器材資訊網

​The method of detecting a primary antibody that is bound to the antigen of interest in an IHC experiment can be either direct or indirect.​ ... In direct detection ... Read More

Direct IF uses a single antibody directed against the target of interest. The primary antibody is directly conjugated to a fluorophore. Indirect IF uses two antibodies ... Read More

2015年1月5日 — Direct IHC. Direct methods are generally one step and involve the use of directly labelled antibodies to detect antigens of interest within the tissue ... Read More

2013年1月29日 — The direct method utilises directly labelled primary antibodies. They may either be purchased already labelled, or can be labelled by the ... Read More

This interaction can then be visualized, directly or indirectly, with a chromogenic reaction catalyzed by reporter enzymes such as Horseradish Peroxidase (HRP) or ... Read More

由 S Shojaeian 著作 · 2018 · 被引用 1 次 — 2. Direct method ... Direct detection methods are known as a one-step process applying a primary antibody, which is directly labeled with reporter molecules, such ... Read More

由 X Chen 著作 · 2010 · 被引用 90 次 — This article summarized immunohistochemistry methods generally used in research laboratories and clinic including direct immune staining, indirect immune ... Read More

The direct method is a one-step staining method and involves a labeled antibody (e.g. FITC-conjugated antiserum) reacting directly with the antigen in tissue ... Read More

Direct method is one step staining method, and involves a labeled antibody (i.e. FITC conjugated antiserum) reacting directly with the antigen in tissue sections. Read More