Immunohistochemistry – direct | 合法醫療器材資訊網

2015年1月5日—DirectIHC.Directmethodsaregenerallyonestepandinvolvetheuseofdirectlylabelledantibodiestodetectantigensofinterestwithinthetissue ...

The aim of this article is to offer definitions and an introduction to the basics methods of immunohistochemistry (or ‘IHC’) which are employed today (and have been used for decades). Continuing the ethos of the previous articles, this article sets out to explain the basics of the main techniques of IHC which should give you an understanding when (inevitably) things go wrong!

IHC can be defined as ‘a localisation of specific antigens in tissue by the use of markers such as fluorescent dyes or enzymes’. IHC isn’t necessarily the end-point, but part of the journey to achieving optimal images. Sample preparation is critical and there is no substitute for production of good tissue sections as a starting point and basis of great IHC and stunning images.

The three main methods of IHC are direct, indirect and enzymatic ‘sandwich’ techniques.

Direct IHC

Direct meth...