Detection of S-phase cell cycle progression using 5 | 合法醫療器材資訊網

由SBBuck著作·2008·被引用261次—Labeling,detection,andquantificationofcellsactivelysynthesizingDNA,orcellsintheS-phaseofcellcycleprogression,arenotonlyimportant ...

Labeling, detection, and quantification of cells actively synthesizing DNA, or cells in the S-phase of cell cycle progression, are not only important in characterizing the basic biology but also in defining the cellular responses to drug treatments, assessing cell health, and determining genotoxicity. The history (1,2) of S-phase cell cycle detection and measurement began in the 1960s with the incorporation of radioactive nucleosides to detect DNA synthesis. The use of autoradiography for analysis was slow and technically difficult. It was subsequently replaced with antibody-based detection of the nucleoside analog 5-bromo-2′-deoxyuridine (BrdU) as the standard method for direct measurement of cells in the S-phase (3). Although an improvement over the older techniques, the typical BrdU assay has limitations. The protocols often require the harsh treatment of DNA using a combination of acid, heat, or nucleases to ...