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EdU cell cycle analysis | 合法醫療器材資訊網

EdU cell cycle analysis

2019年12月4日—BromodeoxyuridineandcellcycleanalysisattheFlowCytometryCoreFacility,InstituteofCellandMolecularScience,BartsandTheLondon, ...

 EdU cell cycle analysis

S-phase analysis using 5-ethynyl-2 deoxyuridine (EdU) uptake

EdU like BrdU is a nucleoside analogue to thymidine which is actively transported into cells while dividing and is incorporated into the newly synthesized DNA structure. The use of antibodies with a fluorescent tag against BrdU makes it possible to determine S phase if cells were pulsed with 10uM BrdU more accurately then just using DNA binding dyes such as PI, 7-AAD, DAPI or ToPro-3. However the method by a practiced technician takes 2 hours and requires the harsh treatment of cells with 2M hydrochloric acid. Thus making it difficult to detect surface and other intracellular antigens when using the standard BrdU protocol.

When using EdU (10 uM) to determine S phase the labelling procedure takes 45 minutes. This involves an aldehyde fixation step for 15 mins. After which a permeabilisation step for antibody labelling of surface or int...

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EdU cell cycle analysis | 合法醫療器材資訊網
2019年12月4日 — Bromodeoxyuridine and cell cycle analysis at the Flow Cytometry Core Facility, Institute of Cell and Molecular Science, Barts and The London, ... Read More
Quantification of cell cycle kinetics by EdU (5 ... | 合法醫療器材資訊網
由 PD Pereira 著作 · 2017 · 被引用 24 次 — Parameters on cell cycle phases can then be estimated from the time required for BrdU-labelled cells to reach M phase, yielding absolute G2 duration, and from ... Read More
Quantification of cell cycle kinetics by EdU (5 ... | 合法醫療器材資訊網
由 PD Pereira 著作 · 2017 · 被引用 24 次 — Quantification of cell cycle kinetics by EdU (5-ethynyl-2'-deoxyuridine)-coupled-​fluorescence-intensity analysis. Oncotarget. 2017 Jun 20;8(25):40514-40532. Read More
BrdUEdU dual labeling to determine the cell | 合法醫療器材資訊網
由 L Harris 著作 · 2018 · 被引用 12 次 — Measuring the mean duration of synthesis-phase (T s ) and of the total cell-cycle (​T c ) within progenitor cell populations can provide important ... Read More
Detection of S | 合法醫療器材資訊網
由 SB Buck 著作 · 2008 · 被引用 261 次 — The 5-bromo-2'-deoxyuridine (BrdU) labeling of cells followed by antibody staining ... Described is an improved method for the detection of S-phase cell cycle ... Read More
Detection of S-phase cell cycle progression using 5 | 合法醫療器材資訊網
由 SB Buck 著作 · 2008 · 被引用 261 次 — Labeling, detection, and quantification of cells actively synthesizing DNA, or cells in the S-phase of cell cycle progression, are not only important ... Read More
Quantification of cell cycle kinetics by EdU (5 ... | 合法醫療器材資訊網
由 PD Pereira 著作 · 2017 · 被引用 24 次 — Keywords: cell cycle, EdU, S phase, DNA replication. Received: May 20, 2016 Accepted: April 03, 2017 Published: April 15, 2017. ABSTRACT. Read More
細胞週期分析Cell Cycle Analysis | 合法醫療器材資訊網
細胞週期分析Cell Cycle Analysis ... 流式細胞儀是現代生物學、生物醫學、腫瘤學、血液學、免疫學、藥理學與臨床研究等領域中，不可或缺的利器。流式細胞儀上樣 ... Read More
A rapid and robust assay for detection of S | 合法醫療器材資訊網
由 E Kotogány 著作 · 2010 · 被引用 162 次 — Progress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells ... Read More
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