DNA extraction | 合法醫療器材資訊網

Thefirstisolationofdeoxyribonucleicacid(DNA)wasdonein1869byFriedrichMiescher....mixturepriortotheDNA-precipitation.Afterisolation,theDNAisdissolvedinaslightlyalkalinebuffer,usuallyinaTEbuffer,orinultra-purewater.

Process of isolating DNA from cells

The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher.[1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue . It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components. The purified DNA can then be used for downstream applications such as PCR,[2] sequencing, or cloning. Currently, it is a routine procedure in molecular biology or forensic analyses.

This process can be done in several ways, depending on the type of the sample and the downstream application,[3] the most common methods are: mechanical, chemical and enzymatic lysis, precipitation, purification, and concentration. The specific method used to extract the...