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DNA Extraction and Purification | 合法醫療器材資訊網

DNA Extraction and Purification

Commerciallyavailablekitsdifferinavarietyofways,includingthecomponentsofthelysisbuffer,themethodusedtoseparateDNA,thetypeofmembraneused,​ ...

 DNA Extraction and Purification

Introduction

Basic considerations

DNA extraction is required for a variety of molecular biology applications. Figure 1 lists the basic steps involved in all DNA extraction methods. Many commercial kits are available to isolate DNA from a variety of biological materials [1, 2]. The sensitivity of polymerase chain reaction (PCR) detection has been shown to be different for various DNA kits [3]. Therefore, selecting the best methodology for your application is crucial.

Figure 1. Basic steps involved in all DNA extraction methods.

Choosing the correct DNA extraction kit can save crucial time on optimization and execution of the experiment. Factors to be considered for selecting a kit include:

Sample origin: Different kits are used to extract material from specific sources, including human tissues, blood, hair, rodent tissues, leaf tissue, bacteria, yeast, fungi, insect, stool, body fluids, spores, soil, clinical samples (e.g., biopsy samples, fine nee...

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DNA Extraction from Tissue | 合法醫療器材資訊網
Purified DNA elutes instantly into this elution buffer, and is ready for use in downstream applications. The ChargeSwitch Technology. ChargeSwitch Magnetic ... Read More
Genomic DNA Extraction | 合法醫療器材資訊網
Any residual RNA is removed by digestion with RNase prior to binding samples to the spin column. The lysate is mixed with Binding Buffer (L3) and ethanol to ... Read More
MagMAX™ Cell and Tissue DNA Extraction Buffer | 合法醫療器材資訊網
MagMAX DNA Cell and Tissue Extraction Buffer, a component of the MagMAX DNA Multi-Sample Ultra 2.0 Kit with Cell and Tissue Extraction Buffer, is offered ... Read More
A Modified SDS | 合法醫療器材資訊網
由 VP Natarajan 著作 · 2016 · 被引用 40 次 — Briefly, 0.3 g samples are added to a bead beating tube containing different size glass beads, lysis buffer for rapid and through homogenization ... Read More
DNA extraction protocol | 合法醫療器材資訊網
Solutions: DNA extraction buffer: Contains 0.1 M EDTA @ pH 8, 1% SDS and 200 µg/mL proteinase K. Make a stock ... Read More
KAZU DNA Extraction Buffer | 合法醫療器材資訊網
The KAZU DNA Extraction Buffer allows for the rapid extraction of genomic DNA from plant leaves for the downstream amplification of targets of interest by PCR. Read More
Quick Protocol for Extraction and Purification of Genomic DNA ... | 合法醫療器材資訊網
2019年4月30日 — The elution buffer (10 mM Tris-Cl, pH 9.0, 0.1 mM EDTA) offers strong protection against enzymatic degradation and is optimal for long term ... Read More
DNA Extraction and Purification | 合法醫療器材資訊網
Commercially available kits differ in a variety of ways, including the components of the lysis buffer, the method used to separate DNA, the type of membrane used,​ ... Read More
DNA extraction | 合法醫療器材資訊網
The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. ... mixture prior to the DNA-precipitation. After isolation, the DNA is dissolved in a slightly alkaline buffer, usually in a TE buffer, or in ultra-pure water. Read More
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