A Modified SDS | 合法醫療器材資訊網

由VPNatarajan著作·2016·被引用40次—Briefly,0.3gsamplesareaddedtoabeadbeatingtubecontainingdifferentsizeglassbeads,lysisbufferforrapidandthroughhomogenization ...

Microorganisms are ubiquitously distributed in various marine depositional environments, such as subseafloor continental shelves and hydrothermal deposits along the Mid-Ocean Ridges. The microbial community structure is always a fundamental question in understanding the ecological significance of their activities in biogeochemical elemental cycles. Because most members of the naturally occurring microbial community appeared to be resistant to cultivation in the laboratory (Rappe and Giovannoni, 2003[1]), cultivation-independent molecular techniques, such as PCR-amplification of 16S rRNA genes followed by the high-throughput sequencing, provide us useful information of the microbial community. However, the molecular view of the microbial community could be significantly biased at the initial experimental step due to the incomplete recovery of total environmental DNA from the tar...