Coagulase Test- Principle, Procedure | 合法醫療器材資訊網

2018年6月12日—CoagulasetestisusedtodifferentiateStaphylococcusaureus(positive)whichproducetheenzymecoagulase,fromS.epidermisandS.

Coagulase test is used to differentiate Staphylococcus aureus (positive) which produce the enzyme coagulase, from S. epidermis and S. saprophyticus (negative) which do not produce coagulase. i.e Coagulase Negative Staphylococcus (CONS).

Coagulase is an enzyme-like protein and causes plasma to clot by converting fibrinogen to fibrin. Staphylococcus aureus produces two forms of coagulase: bound and free. 

Bound coagulase (clumping factor) is bound to the bacterial cell wall and reacts directly with fibrinogen. This results in an alternation of fibrinogen so that it precipitates on the staphylococcal cell, causing the cells to clump when a bacterial suspension is mixed with plasma. This doesn’t require coagulase-reacting factor.

Free coagulase involves the activation of ...