Fluorescence In situ Hybridization: Cell | 合法醫療器材資訊網

由CCui著作·2016·被引用85次—Fluorescenceinsituhybridization(FISH)isamacromoleculerecognitiontechnologybasedonthecomplementarynatureofDNAorDNA/RNA ...

Fluorescence in situ hybridization (FISH) uses DNA fragments incorporated with fluorophore-coupled nucleotides as probes to examine the presence or absence of complementary sequences in fixed cells or tissues under a fluorescent microscope. This hybridization-based macromolecule recognition tool was very effective in mapping genes and polymorphic loci onto metaphase chromosomes for constructing a physical map of the human genome (Langer-Safer et al., 1982[1]; Lichter et al., 1993[2]). FISH technology offers three major advantages including high sensitivity and specificity in recognizing targeted DNA or RNA sequences, direct application to both metaphase chromosomes and interphase nuclei, and visualization of hybridization signals at the single-cell level. These advantages increased the analytic resolution from Giemsa bands to the gene level and enabled rapid detection of num...