Fluorescence In Situ Hybridization Fact Sheet | 合法醫療器材資訊網

2020年8月16日—Fluorescenceinsituhybridization(FISH)providesresearcherswithawaytovisualizeandmapthegeneticmaterialinanindividual'scells, ...

Scientists use three different types of FISH probes, each of which has a different application:

Locus specific probes bind to a particular region of a chromosome. This type of probe is useful when scientists have isolated a small portion of a gene and want to determine on which chromosome the gene is located, or how many copies of a gene exist within a particular genome.

Alphoid or centromeric repeat probes are generated from repetitive sequences found in the middle of each chromosome. Researchers use these probes to determine whether an individual has the correct number of chromosomes. These probes can also be used in combination with "locus specific probes" to determine whether an individual is missing genetic material from a particular chromosome.

Whole chromosome probes are actually collections of smaller probes, each of which binds to a different sequence along the len...