Why Cell Cycle Analysis Details Are Critical In Flow Cytometry | 合法醫療器材資訊網

Ethanolfixationispreferredforcellcycleanalysis,andwhilealdehydescanbeused,thecrosslinkingnatureofthesechemicalscanimpairtheDNA ...

The lifecycle of a cell can be described in stages. In diploid cells, much of the time they exist in a resting state, where a cell does what a cell does — such as, undergo differentiation. In some cases, the cells go into a quiescent state, where the level of RNA is reduced. When the appropriate signals are received, cells begin to bulk up and start to replicate the DNA in preparation for division into 2 daughter cells. After the synthesis phase, the cells enter a second period of rest, where everything is checked before the cells undergo mitosis and produce 2 daughter cells. The cycle repeats itself until the cells die. The cell cycle is usually depicted as shown in Figure 1.

Figure 1: The Cell Cycle. Image from Wikipedia[1].

While there are many differences in cells at each stage of the cell cycle, one of the most obvious is the amount of DNA that the cell contains. At the G0 a...