DNA Content for Cell Cycle Analysis of Fixed Cells With ... | 合法醫療器材資訊網

In most cases, cells must be fixed or permeabilized to allow entry of the dye which is otherwise actively pumped out by living cells. For fixation, alcohol or ... Read More

Pre-coat tubes overnight with 2% Bovine Serum Albumin (BSA) in PBS. Vortex gently, slowly adding the cell suspension dropwise to 9 ml of 70% ethanol in a 15 ml ... Read More

This protocol provides information on how to utilize the chemical probe Propidium Iodide (PI) to stain cells after fixation with 70% ethanol. Read More

cells are fixed with 70% ethanol. 2. This method can also be used to assess cell cycle distribution of certain GFP transfected cells. The ethanol fixation ... Read More

5. Wash cells X2 in PBS. (It may be necessary to centrifuge cells at a slightly higher g to pellet after ethanol fixation as the ... Read More

(It may be necessary to centrifuge cells at a slightly higher g to pellet after ethanol fixation.) 6. Add 1 ml of propidium iodide staining solution to cell ... Read More

This protocol uses ethanol to fix and permeabilize cells for staining of DNA in intact cells with propidium iodide (PI). PI staining solutions provided are a ... Read More

Suspend cells in 50 µL of HBSS containing 2% FBS (pipette up and down a few times if your cells readily form aggregates). a. Add 1mL of ICE cold 70% ethanol in ... Read More

Ethanol fixation is preferred for cell cycle analysis, and while aldehydes can be used, the crosslinking nature of these chemicals can impair the DNA ... Read More