Enzyme | 合法醫療器材資訊網

Theenzyme-linkedimmunosorbentassay(ELISA)isanimmunologicalassaycommonlyusedtomeasureantibodies,antigens,proteinsandglycoproteinsin ...

The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum. ELISA assays are generally carried out in 96 well plates, allowing multiple samples to be measured in a single experiment. These plates need to be special absorbant plates (e.g. NUNC Immuno plates) to ensure the antibody or antigen sticks to the surface. Each ELISA measures a specific antigen, and kits for a variety of antigens are widely available.

The ELISA pictured in Figure 1 is what is known as a sandwich ELISA, here two sets of antibodies are used to detect secreted products, e.g. cytokines. The method is stepwise in the order shown.&...