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7-AADisamembraneimpermeantdyethatisgenerallyexcludedfromviablecells.ItbindstodoublestrandedDNAbyintercalatingbetweenbasepairsinG-C-rich ...

Flow cytometry provides a rapid and reliable method to quantify viable cells in a cell suspension. Determination of cell viability is critical when evaluating the physiological state of cells, such as in response to cytotoxic drugs and environmental factors, or during the progression of cancer and other disease states. In addition, it is often necessary to detect dead cells in a cell suspension in order to exclude them from analysis. Dead cells can generate artifacts as a result of non-specific antibody binding or unwanted uptake of fluorescent probes. One method to identify the two cell populations is by dye exclusion. Live cells have intact membranes that exclude a variety of dyes that easily penetrate the damaged, permeable membranes of non-viable cells.

Several different fluorochromes can be used to stain non-viable cells including 7-amino actinomycin D (...