DNA staining for cell cycle | 合法醫療器材資訊網

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由 C Vignon 著作 · 2013 · 被引用 60 次 — Indeed, on one hand 7-AAD is a G-C base-specific DNA intercalator [15] which identifies the sub G1 hypoploid apoptotic cells, and the G0/G1 (2N ... Read More

Commonly used DNA dyes used for flow cytometric cell cycle analysis include ... Fixed Cell Staining Protocols for PI, 7-AAD, ToPro-3 and DAPI. Read More

DAPI. 345. 455. Invitrogen. Hoechst 33342. 350. 461. Invitrogen. DyeCycle Violet*. 369. 437. Invitrogen. Propidium Iodide (PI). 536. 617. Invitrogen. 7-AAD. 546. Read More

7-AAD is a membrane impermeant dye that is generally excluded from viable cells. It binds to double stranded DNA by intercalating between base pairs in G-C-rich ... Read More

Flow cytometry cell cycle analysis using propidium iodide DNA staining. ... An alternative method to allow the DNA dye into the cells is to permeabilize ... Read More

7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in ... Read More

2020年11月5日 — discrimination or 10 ug/mL (1:100 dilution) for cell cycle profiling. ... While 7-AAD staining is retained after formaldehyde fixation, ... Read More

2014年5月21日 — 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and ... Read More