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由ALFSantos著作·2018·被引用7次—DNAwasextractedfromthetailend(2mmlong)ofmice,storedat-20ºC.TheproteinaseKmethod(PKM)andtheKappaExpressExtract®kitwereusedfor ...

Deoxyribonucleic acid (DNA) is the raw material for genetic studies, and therefore laboratory techniques are developed to obtain it with appropriate concentration and integrity.

To compare two methods of DNA extraction regarding sample concentration and integrity.

DNA was extracted from the tail end (2 mm long) of mice, stored at -20ºC. The proteinase K method (PKM) and the Kappa Express Extract® kit were used for extraction. The concentrations and ratios 260/280 and 260/230 were determined by spectrophotometry. DNA integrity was checked on 2% agarose gel with ethidium bromide. For the final test of the extracted samples, a multiplex polymerase chain reaction (PCR) was performed, with primers for the Large gene.

Samples extracted by the PKM presented mean concentration value of 59.4 ± 18.5 ng/µl (260/280 = 1.74 ±...