PCR Primer Design for 16S rRNAs for Experimental ... | 合法醫療器材資訊網

由 F Sambo 著作 · 2018 · 被引用 31 次 — Since the 16S gene sequence is similar but not identical in different organisms, degenerate primers are used for 16S rRNA sequencing. A primer ... Read More

2016年4月26日 — 以下列出幾種常見的幾種16S metagenomics sequencing之library製備方式，以及各自的優點與缺點。 採用Barcoded 16S rRNA primer & Illumina TruSeq DNA ... Read More

由 K Miyazaki 著作 · 2017 · 被引用 11 次 — Primer Design. Nucleotide composition around the 5′- and 3′-end regions of all bacterial 16S rRNA genes (Table 2) is shown in Figure 1 ... Read More

由 S Thijs 著作 · 2017 · 被引用 124 次 — Bacterial taxonomic community analyses using PCR-amplification of the 16S rRNA gene and high-throughput sequencing has become a cornerstone ... Read More

分子中的淡藍色部分為蛋白質，淡橙色部分為RNA單鏈。 16S核糖體RNA（16S ribosomal RNA），簡稱16S rRNA，是原核生物的核糖體 ... Read More

Universal primers — The most common primer pair was devised by Weisburg et al. (1991) and is currently referred to as 27F and 1492R; however, for some ... Read More

由 L Palkova 著作 · 2021 — New studies showed that amplification of different variable regions of 16S rRNA genes may result in different outcome and that results may be ... Read More

由 RY Zhang 著作 · 2020 · 被引用 10 次 — To accurately classify microorganisms, full-length 16S rRNA gene sequences are typically amplified using primer 8F or Arch21 with universal ... Read More

由 F Wang 著作 · 2018 · 被引用 21 次 — To evaluate bacterial 16S rRNA gene primer pairs in regard to bacterial diversity coverage and/or accurate taxonomic assignment, four primers ... Read More