蛋白質純化簡介(中英對照譯文, from | 合法醫療器材資訊網

凝膠電泳是一種常見的實驗室技術，可用於作為製備和分析方法.電泳的原理依賴於在電場中的電荷的離子的移動.在實務上,蛋白質是在含有清潔劑(SDS)的 ...

Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification process may free the protein from a matrix that confines it, separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps may exploit differences in (for example) protein size, physico-chemical properties, binding affinity and biological acti...