FACS buffer composition | 合法醫療器材資訊網

2017年5月12日—5-Addsodiumazide(0.1-1%)...Sodiumazide–atsuitablelowconcentrations–checksbacterialcontamination,preventsphoto-bleachingof ...

2- Supplement with FCS (1-10%) or BSA (0.1-1%)

Serum proteins protect cells from apoptosis, prevent non-specific staining and also prevent cells from sticking to the FACS tubes. However, a high concentration of serum can contribute to auto-fluorescence, therefore the optimum serum level should be pilot tested beforehand.

3- Include EDTA (0.5-5 mM)

EDTA also prevents cation based cell to cell adhesion and so should be included in the buffer if dealing with sticky and adherent cells like macrophages. However, the concentration of EDTA should be titrated first as high levels can be cytotoxic and/or cytocidal.

4- Slip in some DNase I (25-50µg/ml)

Dead cells release their contents including DNA into the medium which can lead to cell clumping and related problems. If the sample has a high percentage of dead cells – ideally, the dead cel...

Sodium azide role in flow cytometry | 合法醫療器材資訊網

Sodium azide acts as a preservative in most antibodies. During flow cytometry it assists in preventing capping, shedding, and internalization of the antibody- ... Read More

Which is the difference between EDTA and Sodium Azide for ... | 合法醫療器材資訊網

I saw for flow cytometry analysis, besides BSA 0.5-1%, the presence of EDTA at 2-3 mM or sodium azide 0.1% in the buffer. I thought to use as buffer 1% BSA and ... Read More

What is difference between staining buffer and FACS buffer? | 合法醫療器材資訊網

2014年6月18日 — Facs buffer is usually used to stain extracellularly but it is generally speaking the buffer that you use to resuspend your cells before and ... Read More

Flow Cytometry 常用buffer 配方 | 合法醫療器材資訊網

FACS buffer (單位：1 L). 88 ml 蒸餾水+ 10 ml 10X PBS + 2 ml FBS（或FCS）. + 0.1 g sodium azide。完全溶解後，儲存於4 ℃冰箱中。整理: 張文瀠. 共一頁. Read More

Stain Buffer (FBS) &mdash | 合法醫療器材資訊網

由 BD Pharmingen 著作 — Aqueous buffered solution containing fetal bovine serum and ≤0.09% sodium azide. Storage Buffer: Description. Stain Buffer (FBS) can be used for the ... Read More

Flow cytometry (FACS) staining protocol (Cell surface staining ... | 合法醫療器材資訊網

Flow cytometry (FACS) staining protocol (Cell surface staining) ... in ice cold FACS Buffer (PBS, 0.5-1% BSA or 5-10% FBS, 0.1% NaN3 sodium azide*). Read More

Flow Cytometry Buffer Recipes | 合法醫療器材資訊網

The buffer contains sodium azide as preservative and animal serum proteins (FBS/BSA) to help minimizing non-specific binding of antibodies. 1x PBS. 2-5 % (v/v) ... Read More

FACS buffer composition | 合法醫療器材資訊網

2017年5月12日 — 5- Add sodium azide (0.1-1%) ... Sodium azide – at suitable low concentrations – checks bacterial contamination, prevents photo-bleaching of ... Read More

Fluorescence Activated Cell Sorting (FACS) | 合法醫療器材資訊網

由 K Nybo 著作 · 2010 · 被引用 1 次 — resuspended them in the FACS wash buffer, which is PBS + 0.1% sodium azide (NaN3), and stained them with my antibodies. All centrifugation steps were done ... Read More

FLOW CYTOMETRY | 合法醫療器材資訊網

Adding sodium azide will prevent the modulation and internalization of ... Ensure adequate washing steps and include tween or triton in wash buffers. Read More

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Sodium azide role in flow cytometry | 合法醫療器材資訊網

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