Flow cytometry (FACS) staining protocol (Cell surface staining ... | 合法醫療器材資訊網

Sodium azide acts as a preservative in most antibodies. During flow cytometry it assists in preventing capping, shedding, and internalization of the antibody- ... Read More

I saw for flow cytometry analysis, besides BSA 0.5-1%, the presence of EDTA at 2-3 mM or sodium azide 0.1% in the buffer. I thought to use as buffer 1% BSA and ... Read More

2014年6月18日 — Facs buffer is usually used to stain extracellularly but it is generally speaking the buffer that you use to resuspend your cells before and ... Read More

FACS buffer (單位：1 L). 88 ml 蒸餾水+ 10 ml 10X PBS + 2 ml FBS（或FCS）. + 0.1 g sodium azide。完全溶解後，儲存於4 ℃冰箱中。 整理: 張文瀠. 共一頁. Read More

由 BD Pharmingen 著作 — Aqueous buffered solution containing fetal bovine serum and ≤0.09% sodium azide. Storage Buffer: Description. Stain Buffer (FBS) can be used for the ... Read More

Flow cytometry (FACS) staining protocol (Cell surface staining) ... in ice cold FACS Buffer (PBS, 0.5-1% BSA or 5-10% FBS, 0.1% NaN3 sodium azide*). Read More

The buffer contains sodium azide as preservative and animal serum proteins (FBS/BSA) to help minimizing non-specific binding of antibodies. 1x PBS. 2-5 % (v/v) ... Read More

2017年5月12日 — 5- Add sodium azide (0.1-1%) ... Sodium azide – at suitable low concentrations – checks bacterial contamination, prevents photo-bleaching of ... Read More

由 K Nybo 著作 · 2010 · 被引用 1 次 — resuspended them in the FACS wash buffer, which is PBS + 0.1% sodium azide (NaN3), and stained them with my antibodies. All centrifugation steps were done ... Read More

Adding sodium azide will prevent the modulation and internalization of ... Ensure adequate washing steps and include tween or triton in wash buffers. Read More