How to…ensure correct calibration | 合法醫療器材資訊網

2018年10月1日—Intheclinicalbiochemistrylaboratory,itisimportanttoappreciateandunderstandthefundamentalprinciplesofcalibrationcurvesand ...

In the clinical biochemistry laboratory, it is important to appreciate and understand the fundamental principles of calibration curves and how to interpret results, as the quality of these determine whether IQC values pass or fail internal rules set by the laboratory.

Two-point linear calibration curves are the most commonly used type of calibration for spectrophotometric methods in the biochemistry laboratory. These calibrations typically use a blank and a known concentration of high standard to construct a straight calibration curve that is subsequently used to calculate the unknown concentration of samples. These linear curves are mathematically determined by Beer-Lambert’s law, this law describes the relationship between the absorbance (A), and three independent variables: molar absorptivity (e), path-length (l), and concentration (c). The law takes the form of y=mx+b, where m is the slope or gradient, b is the y intercept, y is the absorbance and x is the concentration....