Minimum Inhibitory Concentration Test (MIC) | 合法醫療器材資訊網

SummaryoftheMinimumInhibitoryConcentrationTest(MICTest):·ApurecultureofasinglemicroorganismisgrowninMueller-Hintonbrothorotherbrothas ...

Labeled Photo of Microtiter MIC Test –

Labeled Photo of Standard MIC Test –

To do an MIC, one inoculates the test substance with an invisible but high number of microorganisms, then observes the mixture of microorganisms and test substance to see if it changes from clear to cloudy. If it turns cloudy, that means microorganisms have grown to high levels, and the test substance is not inhibitory to them at that particular dilution.

Test wells that remain clear after incubation may contain the original low-level inoculum of viable microorganisms, or the microbes could all have been killed by the antimicrobial agent. Those two outcomes cannot be differentiated visually. For that reason, scientists use MIC assays as indicators of an antimicrobial agent’s inhibitory activity rather than biocidal activity.