Overview of RNase A StructureFunction Relationships | 合法醫療器材資訊網

2010年10月7日—RibonucleaseA(RNaseA)isadigestiveenzymesecretedbythepancreasthatspecificallydigestsorhydrolyzesRNA(butnotDNA) ...

Overview of RNase A Structure/Function Relationships

Ribonuclease A (RNase A) is a digestive enzyme secreted by the pancreas that specifically "digests" or hydrolyzes RNA (but not DNA) polymers by endonuclease cleaveage of the phosphodiester bonds forming the covalent links between adjacent ribonucleotide residues in these molecules.

The relatively small, 124 amino acid sequence of RNase A contains four His residues, two of which -- His12 and His119 are directly implicated as catalytic fresidues of this enzyme. The imidazole ring of His12 has an anonymously low pK value (pK < 6.0) suggesting it must be deprotonated for catalysis. Conversely, the imidazole ring of His119 has an anonymously high pK value (pK > 6.0) suggesting it must be protonated for catalysis.

Click here[1] to view the microenvironments surround the of th...