Staining Cell Surface Targets for Flow Cytometry | 合法醫療器材資訊網

Read More

Note: This protocol is intended for use with the specific products mentioned within it. Substituting different products is not recommended. Introduction. Cell surface ... Read More

Protocol C: Two-step protocol for Fixation/Methanol — The following protocol allows the simultaneous analysis of cell surface molecules and ... Read More

This protocol provides an overview of how to generally perform flow cytometric staining, including steps on tissue/sample harvesting, red blood cell lysis, Fc ... Read More

By staining cell surface markers, researchers can identify specific cell populations and perform fluorescence-activated cell sorting (FACS). The following flow ... Read More

Staining Cell Surface Targets for Flow Cytometry ... Protocol A: Cell Suspensions ... Except where noted in the protocol, all staining should be done on ice or at ... Read More

We recommend staining with ice-cold reagents/solutions and at 4°C, as low temperature and presence of sodium azide prevent the modulation and internalization ... Read More

There are many protocols for staining cells for flow cytometry. Protocols may to need be optimized for different cell types, targets, or applications. This is our basic ... Read More

Resuspend the cell pellet in either 200-µL (for microwell plates) or 0.5-mL (for tubes) volumes of Stain Buffer. Analyze stained cell samples by flow cytometry. Read More

Flow Cytometry Protocol: Cell Surface Marker Staining · PBS: Dissolve 8 g NaCl, 0.2 g KCl, 1.15 g Na2HPO4 and 0.2 g KH2PO4 in 800 mL distilled water. · Cell ... Read More