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Note:Thisprotocolisintendedforusewiththespecificproductsmentionedwithinit.Substitutingdifferentproductsisnotrecommended.Introduction.Cellsurface ...

Note: This protocol is intended for use with the specific products mentioned within it. Substituting different products is not recommended.

Cell-surface markers can be used to define cell subsets based on lineage and developmental stage, as well as function when they are labeled with fluorochrome-conjugated antibodies and analyzed by flow cytometry. These surface markers have different forms and functions and include receptors for both soluble and cell-bound ligands, ion channels, glycoproteins, phospholipids, and more. For example, CD4 is a surface marker for T helper cells that can be further differentiated based on expression of other chemokine receptors and cluster of differentiation (CD) markers. Live cells stained with antibodies can be sorted based on unique staining p...