How can I cross link proteins using glutaraldehyde? | 合法醫療器材資訊網

由 I Migneault 著作 · 2004 · 被引用 1610 次 — No single mechanism seems to be responsible for glutaraldehyde crosslinking with proteins. All reported forms of glutaraldehyde exhibit the ... Read More

Depending on you result, you can narrow down the % of glutaraldehyde in your reaction to look for the optimum % needed. . ie: 0.005%, 0.01% or something like​ ... Read More

Glutaraldehyde is an aggressive carbonyl (–CHO) reagent that condenses amines via Mannich reactions and/or reductive amination. It is an indiscriminant ... Read More

由 DT Cheung 著作 · 1982 · 被引用 154 次 — reported the isolation of a pyridinium type compound following the reaction of glutaraldehyde with amines and suggested this struc- ture as the stable crosslink. Read More

由 M Salem 著作 · 2010 · 被引用 48 次 — The glutaraldehyde cross-link is known to be a highly nonspecific process that involves most of the lysine residues distributed on the surface of ... Read More

由 M Kapoor 著作 · 被引用 19 次 — proteins are available, chemical crosslinking is the ideal strategy for an ... Glutaraldehyde, a popular reagent, has been used in a variety of applications where. Read More

To help overcome its tendency to form large-molecular-weight polymers upon crosslinking two proteins, a two-step protocol often is employed. In this regard, one ... Read More

由 JM Ruijgrok 著作 · 1994 · 被引用 44 次 — Experiments were carried out to study the effect on the degree of crosslinking of: (​a) short term (1 or 5 min) high (50°C temperature glutaraldehyde (GA) fixation ... Read More

由 DT Cheung 著作 · 1982 · 被引用 250 次 — When dilute collagen solutions were reacted with low concentrations of glutaraldehyde, intramolecular crosslinks were observed as the predominant crosslinks. Read More