Performance of a fluorescence polarization immunoassay for ... | 合法醫療器材資訊網

由SHMastin著作·1993·被引用15次—TheteicoplaninFPIAusessixserum-basedcalibratorsovertherange0-100micrograms/ml,withthelowestteicoplaninconcentrationat5micrograms/mlanda ...

Teicoplanin is a new glycopeptide antibiotic that is structurally related to vancomycin, but has six major components and is > 90% protein bound. We have developed a competitive homogeneous fluorescence polarization immunoassay (FPIA) for the quantitative determination of teicoplanin in serum. The teicoplanin FPIA uses six serum-based calibrators over the range 0-100 micrograms/ml, with the lowest teicoplanin concentration at 5 micrograms/ml and a lower limit of detection of < 1.5 micrograms/ml. Samples with concentrations > 100 micrograms/ml can be analyzed after 1:5 dilution with serum. Intra- and interassay precision are < 2% and < 4.5%, respectively, for controls at 7.5, 35, and 75 micrograms/ml on an automated FPIA analyzer. Recovery results for 30 samples prepared from the teicoplanin analytical reference standard over the range 3.4-358 micrograms/ml were linearly correlated with target concentr...