FPIA | 合法醫療器材資訊網

Fluoresencepolarizationimmunoassay(FPIA)isalsoahomogenouscompetitiveimmunoassay.Inthissystem,fluorescein-labeleddrugcompeteswith ...

Fluoresence polarization immunoassay (FPIA) is also a homogenous competitive immunoassay. In this system, fluorescein-labeled drug competes with unlabeled drug from the patients serum sample for binding sites on an antibody reagent.

The patients sample, presumably containing the therapeutic drug that is being monitored, and the fluorescein-labeled drug are added to a chamber containing antibody for that drug.

The labeled and unlabeled drug will compete for binding sites on the antibody. The greater the amount of drug in the sample, the fewer the number of binding sites that are available for the labeled analyte, leaving a greater number of small, free fluorescein-labeled molecules in the solution.

When the chamber is excited with plane polarized light, fluorescein will absorb the light and emit it at a higher wavelength as fluorescent light.

A small, free fluor...