Harvesting Cells for Flow Cytometry | 合法醫療器材資訊網

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Protocol A: Tissue culture cells · 60 x 15 mm tissue culture dish; 3-mL syringe or frosted glass microscope slides; Cell strainer (nylon mesh) · Scissors or scalpel ... Read More

Harvest the cells (if obtaining from tissue), decant (if grown in the flask) and centrifuge them for 4-5 minutes (300-400xg) at 4°C and discard the supernatant. Read More

Preparation of adherent tissue culture cell lines · Harvest cells by enzymatic release using 1x Accutase solution or 0.25% trypsin, followed by quenching with media ... Read More

For adherent cell populations, wash cells (similar to a media exchange) in flow cytometry staining buffer and harvest cells by gently scraping the dish, plate, ... Read More

2013年7月30日 — Protocol A: Cell Preparation of Tissue Culture Cells ... Collect cells in 10 mL of Flow Cytometry Staining Buffer and pass cell suspension. Read More

2013年3月4日 — Soft tissues like spleen or liver, for example, can be rubbed between two etched glass slides in a circular motion, or mashed through a nylon ... Read More

They can be pretty tricky to get into single cell suspension. You could try using UpCell plates (Nunc) that don't require any enzyme to release the cells from the ... Read More

2013年7月30日 — Protocol A: Cell Preparation of Tissue Culture Cells ... Collect cells in 10 mL of Flow Cytometry Staining Buffer and pass cell suspension. Read More

General procedure for flow cytometry using a conjugated primary antibody. · Harvest, wash the cells, and adjust cell suspension to a concentration of 1-5 x 10​6 ... Read More