Protocol | 合法醫療器材資訊網

Experience challenges in harvesting cells from cell lines? Here is a protocol for efficient harvesting of cells from tissue culture.

Single cells must be suspended at a density of 105-107 cells/ml to keep the narrow bores of the flow cytometer and its tubing from clogging up. The concentration also influences the rate of flow sorting, which typically progresses at 2,000-20,000 cells/second. Phosphate Buffered Saline (PBS) is a common suspension buffer.

The most straight forward samples for flow cytometry are non-adherent cells from tissue cell culture. Here we describe methods for both tissue culture cell lines and adherent tissue culture cell lines.

Reagents PBS/BSA (PBS containing 1% Bovine Serum Albumin (BSA)) PBS Accutase solution 1X Trypsin 0.25% Cells stored in liquid nitrogen Prepare cold PBS/BSA buffer. Careful...

Protocol | 合法醫療器材資訊網

BestProtocols | 合法醫療器材資訊網

Protocol A: Tissue culture cells · 60 x 15 mm tissue culture dish; 3-mL syringe or frosted glass microscope slides; Cell strainer (nylon mesh) · Scissors or scalpel ... Read More

Flow Cytometry | 合法醫療器材資訊網

Harvest the cells (if obtaining from tissue), decant (if grown in the flask) and centrifuge them for 4-5 minutes (300-400xg) at 4°C and discard the supernatant. Read More

Preparation Of Tissue Culture Cells Protocol | 合法醫療器材資訊網

Preparation of adherent tissue culture cell lines · Harvest cells by enzymatic release using 1x Accutase solution or 0.25% trypsin, followed by quenching with media ... Read More

Step-By | 合法醫療器材資訊網

For adherent cell populations, wash cells (similar to a media exchange) in flow cytometry staining buffer and harvest cells by gently scraping the dish, plate, ... Read More

Cell Preparation for Flow Cytometry | 合法醫療器材資訊網

2013年7月30日 — Protocol A: Cell Preparation of Tissue Culture Cells ... Collect cells in 10 mL of Flow Cytometry Staining Buffer and pass cell suspension. Read More

Harvesting Cells for Flow Cytometry | 合法醫療器材資訊網

2013年3月4日 — Soft tissues like spleen or liver, for example, can be rubbed between two etched glass slides in a circular motion, or mashed through a nylon ... Read More

How do I harvest adherent cells (MCF | 合法醫療器材資訊網

They can be pretty tricky to get into single cell suspension. You could try using UpCell plates (Nunc) that don't require any enzyme to release the cells from the ... Read More

Cell Preparation for Flow Cytometry | 合法醫療器材資訊網

2013年7月30日 — Protocol A: Cell Preparation of Tissue Culture Cells ... Collect cells in 10 mL of Flow Cytometry Staining Buffer and pass cell suspension. Read More

Direct flow cytometry (FACS) protocol | 合法醫療器材資訊網

General procedure for flow cytometry using a conjugated primary antibody. · Harvest, wash the cells, and adjust cell suspension to a concentration of 1-5 x 106 ... Read More

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