PMSF | 合法醫療器材資訊網

Inbiochemistry,phenylmethylsulfonylfluoride(PMSF)isaserineproteaseinhibitor(serinehydrolaseinactivator)commonlyusedinthepreparationofcell ...

From Wikipedia, the free encyclopedia

Chemical compound

In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate all serine proteases.[1] The effective concentration of PMSF is between 0.1 - 1 mM. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C).[2] At 4˚C, pH 8, PMSF is almost completely degraded after 1 day.[2] Stock solutions are usually made up in anhydrous ethanol, isopropanol, or corn oil and diluted immediately before use.

PMSF reacts specifically with the active site serine residue in serine hydrolases. It does not bind to any other serine residues in the protein. This is a result of the hyperactivity of that ...