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苯甲基磺醯氟 | 合法醫療器材資訊網

苯甲基磺醯氟

PMSF在水中會迅速降解，所以一般會用無水酒精，異丙醇或者二甲亞碸（DMSO​）...Inactivationoftheproteaseinhibitorphenylmethylsulfonylfluorideinbuffers.

 苯甲基磺醯氟

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苯甲基磺醯氟（phenylmethanesulfonylfluoride or phenylmethylsulfonyl fluorid，PMSF）是一種絲氨酸蛋白酶抑制劑，在生物化學領域，常被用來製備細胞裂解液。PMSF在水中會迅速降解，所以一般會用無水酒精，異丙醇或者二甲亞碸（DMSO）來製備儲備溶液。一般PMSF執行蛋白水解抑制作用的濃度為0.1-1mM。在水溶液中的半衰期短（在pH7下為110分鐘，在pH7.5下為55分鐘，在pH8下為35分鐘，全部在25℃下）[1]。

PMSF會特異性結合到絲氨酸蛋白酶的活性絲氨酸殘基，但不會結合到非活性絲氨酸殘基。

參考資料[編輯] 外部連結[編輯]

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