iFISH is a publically available resource enabling versatile ... | 合法醫療器材資訊網

由EGelali著作·2019·被引用21次—Atpresent,thereisalackoffreeresourcesofDNAFISHprobesandprobedesigntoolswhichcanbereadilyapplied.Here,wedescribeiFISH, ...

We developed the iFISH4U probe design web interface (http://ifish4u.org/probe-design/[1]) with a strong focus on versatility, as a Python3 package named ifpd. The interface allows a user to run one of two types of query: ‘single query’ or ‘spotting query’. A single query (ipfd_query_probe) produces a list of potential single-locus probe candidates, each comprising N oligos, in a given genomic region of interest. To achieve this, it first identifies all possible sets (herein, referred to as probe candidates) of N oligos in the region of interest. Then, for each probe candidate, the interface computes three features: (i) size, (ii) centrality, and (iii) homogeneity. We defined the probe size as the difference between the genomic coordinates of the first base covered by its first oligo, and the last base covered by its last oligo. We defined the centrality Cp(X) of a prob...