Standards for total serum protein assays | 合法醫療器材資訊網

由BTDoumas著作·1975·被引用392次—Standardsolutionswerepreparedfromlyophilizedpreparationsofhumanserumalbuminandbovineserumalbumin,withcorrectionsmadeforvolatilematerial ...

We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serum albumin, with corrections made for volatile material and ash contents. These solutions and a solution of crystalline albumin standard were analyzed with a new stable biuret reagent, to establish absorptivity values (values for the absorbance of a 1 g/liter final reaction mixture). The mean values obtained were 0.302, 0.292, and 0.290 for human serum albumin, bovine serum albumin, and the crystalline albumin, respectively. We believe that the established absorptivity value will improve the accuracy of serum protein determinations. We studied the linearity of the relation between color produced and protein concentration, with use of the solutions described above and a serum pool. The color adheres to Beers law up to the highest...