Immunohistochemistry (IHC) | 合法醫療器材資訊網

ThemostpopularcounterstainusedwithchromogenicIHCstainingishematoxylin,whichstainsnucleiblue,contrastingwiththebrownofHRP-DAB.Hematoxylin ...

Discover your comprehensive guide to immunohistochemistry (IHC). From tissue processing and antibody selection to detection, controls, and troubleshooting, this guide will help boost your research.

Immunohistochemistry (IHC) uses antibodies to detect the location of proteins and other antigens in tissue sections. The antibody-antigen interaction is visualized using either chromogenic detection with a colored enzyme substrate, or fluorescent detection with a fluorescent dye.

Although less quantitative than assays such as western blotting or ELISA, IHC gives invaluable information about protein localization in the context of intact tissue. Protein expression patterns are tremendously valuable for pathologists and as diagnostic tools.

Essential to a successful IHC experiment is a robust, optimized, and reproducible stain...