Intro to Immunohistochemistry (IHC) Staining | 合法醫療器材資訊網

AnIntroductiontoIHCStaining...IHCisusedinhistologytodetectthepresenceofspecificproteinmarkersthatcanassistwithaccuratetumorclassificationand ...

A simple, but sometimes overlooked step is to choose antibodies that work for immunohistochemistry. This can save you a lot of headaches down the road. Search for specific antibodies from literature, vendors, as well as your peers.

There are several things to keep in mind with antibodies. What might work well in one laboratory might not be optimal for your laboratory. Each antibody needs to be tested with your staining system. Antibodies over time can lose their staining intensity. Exposure to air and light can cause this.

When selecting antibodies, there are two main options to consider; a concentrated format or a pre-diluted, Ready-To-Use (RTU) format. Concentrates are flexible, have a lower initial purchase price, and can generally be used within any staining system, both automated and manual, subject...