Platelet Activation | 合法醫療器材資訊網

Theinvestigatorhasseveraloptionswhenperformingplateletassaysbyflowcytometry.Anticoagulant,bloodcollection,fixation,agonist,andgating/analysis ...

This procedure describes the preparation of fresh or fixed, unstimulated or in vitro activated platelets from unlysed whole blood; staining of platelets; and setting up the FACS brand flow cytometer prior to acquisition of platelet data. When performing assays to detect in vivo platelet activation or analyzing in vitro platelet activation, it is critical that artifactual stimulation of the platelets be minimized during blood collection and sample handling. Even with careful preparation, low levels of inadvertent platelet activation can be difficult to avoid. Therefore, nominally unstimulated platelets should always be analyzed in parallel with in vitro stimulated samples to measure specific effects. The investigator has several options when performing platelet assays by flow cytometry. Anticoagulan...